A modular approach towards the synthesis of target-specific MRI contrast agents

In this paper, a modular pathway towards the synthesis of new specific MRI contrast agents and their luminescent analogues is described. Using two azide bearing probes: 3β-deoxycholic acid and cRGDfK(N3), we performed a “click” reaction with an alkyne bearing DOTA analogue, yielding a possible blood-pool contrast agent, as well as a contrast agent with a high affinity for αvβ3 integrin expressing cells. The NMRD profiles and HSA titration of the deoxycholic acid bearing contrast agent show that this contrast agent interacts with HSA. T1 measurements of Jurkat T cells in the presence of the contrast agent, proved the retention of integrin binding of the RGD bearing contrast agent.status: publishe