Interphotoreceptor retinoid-binding protein as biomarker in systemic autoimmunity with eye inflictions

Autoimmune diseases of the eye, exemplified by Behçet disease and Vogt-Koyanagi-Harada disease, are a major cause of blindness. We studied interphotoreceptor retinoid binding protein (IRBP), a dominant autoimmune antigen in the eye. Aqueous humor samples from 28 patients with active uveitis were analysed for immunoglobulin G (IgG) content as a marker for blood-ocular barrier breakdown and by gelatinase B zymography for the detection of inflammation. The data were correlated with the presence of intact IRBP ( asymptotically equal to 140 kD) as determined by Western blot analysis and with the clinical disease activity. Aqueous humor samples from control eyes and eyes with low disease activity showed positive immunoreactivity for intact IRBP. The IRBP signal weakened or disappeared with higher disease activity. Significant positive correlations were observed between disease activity and levels of matrix metalloproteinase-9 (r(s)= 0.713; p<0.001) and IgG (r(s)= 0.580; p = 0.001). Significant negative correlations were found between levels of IRBP and disease activity (r(s)= -0.520; p = 0.005) and levels of MMP-9 (r(s)= -0.727; p<0.001) and of IgG (r(s)= 0.834; p