3D nanoscopy: Bringing biological nanostructures into sharp focus

(Figure Presented) Zooming in: Ultrahigh-resolution microscopy is developing rapidly by different approaches. Photoactivation-localization microscopy (PALM) can now provide 3D images with fast acquisition times through the use of photoactivatable rhodamines and two-photon excitation. In PALM, individual emitters can be localized with nanometer precision simply by determining the centroid of the emission distribution. © 2007 Wiley-VCH Verlag GmbH & Co. KGaA.status: publishe