Digestibility of a complete ration in horses fed once or three times a day and correlation with key blood parameters

To determine the influence of feeding frequency on apparent digestibility and blood metabolites in horses, four geldings were fed a complete ration either once (at 08:00 h) or three times a day (at 08:00, 13:00 and 18:00 h). Horses were provided with a mixture of cereals and chopped alfalfa hay at maintenance level for energy supply, 344 kJ/kg BW0.75 on a daily basis. After three weeks' adaptation, total amounts of faeces and urine were collected for five days, using collection harnesses. Serial blood samples were taken at -30, -15, 0, 5, 15, 30, 45, 60, 90, 120, 180, 240, 300, 360, 480 and 600 min after feeding and analysed for glucose, L-lactate, triglycerides (TG), non-esterified free fatty acids (NEFA) and triiodothyronine (TAstatus: publishe