Incorporation of a disaccharide nucleoside into the backbone of double-stranded DNA: crystallization and preliminary X-ray diffraction

Incorporation of a disaccharide nucleoside into double-stranded DNA can be considered as a chemical (non-enzymatic) alternative for site-specific cleavage of DNA. Crystals of the sequence d(CGCGAATT*CGCG), where * is an incorporated ribose, were obtained by hanging-drop vapour diffusion and diffracted to 2.6 A. The crystals belong to the orthorhombic space group P222(1), with unit-cell parameters a = 41.52, b = 57.63, c = 81.39 A, indicating a new crystal packing motif for an oligonucleotide dodecamer sequence.journal: Acta Crystallographica Section F: Structural Biology and Crystallization Communications content_type: crystallization communications peer_reviewed: Yes review_process: Single blind received: 13 September 2005 accepted: 22 September 2005 published_online: 30 September 2005 copyright: © 2005 International Union of Crystallographystatus: publishe