EFEK SCAFFOLD BOVINE HYDROXYAPATITE-GELATIN-ALENDRONAT PADA PENUTUPAN DEFEK TULANG DAN EKSPRESI ALKALI FOSFATASE DI TULANG

The main purpose of this study was to examine the effect of Bovine Hydroxyapatite-Gelatin-Alendronate (BHA-Gel-Ale) scaffold on the healing process of the defect and expression of Alkaline Phosphatase (ALP) on defect model. This study used 36 male Wistar strain rats that divided into 3 groups, namely positive control, BHA-Gel and BHA-Gel-Ale. Defect models were made by drilling the femur with a diameter of 2 mm in all groups. In the positive control group, the defect was not given a scaffold, in the BHA-Gel group the defect was given a BHA-Gel scaffold, and BHA-Gel-Ale scaffold for the BHA-Gel-Ale group. On the 14th and 28th day termination was performed to take the treated femur. Radiological analysis and ALP expression analysis with Immunoreactive Score (IRS) in the Immunohistochemistry (IHC) test was performed. In the 14th and 28th day of radiological analysis, closure of the defect in BHA-Gel-Ale group was the worst among all groups. Furthermore, ALP expression in the BHA-Gel group was significantly higher than the positif control group (p0,05). This study shows that BHA-Gel-Ale scaffold cross-linked with GA 0.5% does not improve the repairment of bone defects and ALP expressio