AKTIVITAS ANTIHEPATITIS C DARI KOMBINASI KSTRAK ETANOL DAUN Ruta angustifolia L. DENGAN OBAT ANTIVIRUS HEPATITIS C SECARA IN VITRO

Ruta angustifolia L. is a plant of rutaceae’s family which has been traditionally used forjaundice. Previous study showed that ethanol extract of R. angustifolia inhibit hepatitis C virus against JFH1a virus on hepatocyte cell Huh7 with IC50 3.0 ± 1.4 μg/ml. In order to continue our study, ethanol extract of R. angustifolia combined with antihepatitis C virus drugs such as simeprevir, telaprevir, and ribavirin to determine the activities and synergistic or additive effects caused by these combinations. The results of the combination activity of extract R. angustifolia with simeprevir obtained the increasing of antihepatitis C virus activity which is showeda decreasing IC50 simeprevir, which is from 43.84 ± 0.96 nM (single treatment) to 19.70 ± 0.28 nM (combination) with a combination index value (CI) = 0.833. The combination of extract R. angustifolia with telaprevir decreased IC50 telaprevir from 10.48 ± 0.11 nM (single treatment) to 3.64 ± 0.07 nM (combination) and CI value = 0.677. Then a combination of extract R. angustifolia with ribavirin which can decrease IC50 ribavirin from 10.43 ± 0.18μg/ml (single treatment) to 2.80 ± 0.03 μg/ml (combination) with CI value = 0.691. The Compusyn software possess a CI value