Daya Antibakteri Ekstra Etanol Daun Beluntas (Pluchea indica less) Terhadap Escherichia coli Secara In Vitro

The aim of this study was to investigate the antibacterial activity of the ethanol extract Pluchea indica less leaves against Escherichia coli by in vitro. The method using broth dilution test was determined Minimum Bactericidal Concentration (MBC) with a broth extract cultures into Eosin Methylen Blue Agar (EMBA) medium. The twice tubes of concentration extract of 2546 and 5046 was showed no visible turbidity, and then inoculated into Eosin Methylen Blue Agar medium. After 24 h of incubation at 37 oC, Minimum Bactericidal Concentration (MBC) was determined which no viable growth of Escherichia coli at Eosin Methylen Blue Agar (EMBA) medium. The result showed that Minimum Bactericidal Concentration (MBC) of the extract of 259p and 5040 had been similar (X2x0.05) on the growth of Escherichia coli. Based on this result, Minimum Bactericidal Concentration (MBC) of the extract was used 2546 concentration. Key words : antibacterial, Pluchea indica less, Escherichia col