17-Hydroxyprogesterone responses to gonadotrophin-releasing hormone agonist buserelin and adrenocorticotrophin in polycystic ovary syndrome: Investigation of adrenal and ovarian cytochrome P450c17 alpha dysregulation

Abnormal regulation of cytochrome P450c17 alpha causes the exaggerated secretion of ovarian androgens in polycystic ovary syndrome (PCOS). This enzyme is active in both the ovaries and adrenal glands. We examined whether there is an abnormal regulation of cytochrome P450c17 alpha in the adrenal gland by investigating the relationship of 17-hydroxyprogesterone (17-OH progesterone) hyperresponsiveness to the gonadotrophin releasing hormone (GnRH) agonist, buserelin, testing with 17-OH progesterone response to adrenocorticotrophic hormone (ACTH) in PCOS, In all, 68 women with PCOS and 24 normal women were included in the study, Ultrasound, clinical and hormonal parameters were used to define PCOS, 17-OH progesterone response to ACTH was measured in all the women. In 52 of the 68 women with PCOS, 17-OH progesterone response to buserelin was measured, The mean basal 17-OH progesterone concentrations were similar in both PCOS and control groups, PCOS n omen had significantly higher net increment in 17-OH progesterone after ACTH administration (P