preparation of maltase-inorganic hybrid nanoflowers and investigation of inflence on enzymatic activity of synthesis conditions

Enzymes have found widespread in variousscientific and technical fields, including chemistry, biochemistry, medicine,pharmaceutical science and industry [1]. Although enzymes have uniqueproperties, such as high catalytic activity, reaction specificity, stability,selectivity, low toxicity and water solubility, their efficient use isvery limited due to their innate disadvantages associated with instabilityduring storage, loss of catalytic activity after  implementationinto the reaction, high production cost and lack of recovery from reactionmedium, substrate- and product-inhibitions [2].  In order to overcome these limitations,several immobilization approaches have been developed.Inthis study,  maltase-copper phosphate hybrid nanoflowers(HNFs) synthesized and at the same time formation mecanism, catalytic activityand stability under different experimantal parameters (pHs, temperatures) wasinvestigated. ThehNFs were characterized using SEM, EDX analysis, Bradford assay, UV-Visspectrometry and XRD. The effect of reaction temperatures on the morphology ofthe hNFs was demonstrated with SEM images (Fig. 1).