Determination of acute and early life stage toxicity of fat-plant effluent using zebrafish (Danio rerio)

The present study examines the effects of an effluent from a fat plant (FP) on zebrafish (Danio rerio) embryos and larvae using the whole effluent toxicity testing methods (WET). The method is based on acute toxicity using 96-h larval mortality and chronic toxicity using endpoints such as the time to hatch, hatching success, deformity, growth rate, swim-up failure, accumulative mortality, and sex ratio. On the basis of larval mortality the 96-h LC50 (the concentration was lethal to 50% of newly hatching zebrafish larvae) was 68.9%. In chronic toxicity test, newly fertilized embryos (