Determination of proteolytic and lipolytic activities of some pseudomonas bacteria

In this study, a total of fifteen strains belonging to Pseudomonas fluorescens ssp. indologenes, Pseudomonas vesicularis, Pseudomonas luteola, Pseudomonas aeruginosa were analysed. Strains were isolated from the raw milk and identified by using Analytical Profile Indeks (API 20 NE). Antibiotic susceptibility tests of Pseudomonas spp. strains to ampicillin (10 µg), amikacine (30 µg), gentamicine (10 µg), oflaxacine (5 µg), tetracikline (30 µg), chloramphenicol (30 µg), cephuroxime (30 µg) were determined by using the disk diffusion method. The results were described according to NCCLS standards. It was determined that 57% of Pseudomonas strains were susceptibility, 35% were resistant and 7% were intermediate susceptibility against fifteen antibiotics tested. Proteolytic activities of Pseudomonas strains were tested in Skim Milk Agar (SMA) medium and fourteen strains were determined that had a proteolytic activities. Proteolytic activities of these strains were found between 8.0 and 55.0 mm with an average 28.1 mm zone diameter. The highest proteolytic activity 55.0 mm was found in P. fluorescens ssp. indologenes T7 strain. In this study, lipolytic activities of Pseudomonas strains were tested in Tributirin Agar (TA) medium. Thirteen strains were determined that had a lipolytic activities. Lipolytic activities of these strains were found between 5.3 and 29.3 with an average 15.4 mm zone diameter. Zone diameter of the highest lipolytic activity 29.3 mm was found in P. fluorescens ssp. indologenes T8 strain. Our research in this sense, purification and economic benefits of microbial enzymes, which are used in the pharmaceutical industry, sheds lightKey words : Disk diffussion , Pseudomonas strains, Proteolytic activity, Lipolytic activit