Polypeptide with electroactive endgroups as sensing platform for the abused drug ‘methamphetamine’ by bioelectrochemical method

PubMed ID: 27769482Affinity-type sensors have emerged as outstanding platforms in the detection of diagnostic protein markers, nucleic acids and drugs. Thus, these novel platforms containing antibodies could be integrated into the monitoring systems for abused drugs. Herein, we established a novel detection platform for the analysis of a common illicit drug; methamphetamine (METH). Initially, a fluorescent-labeled polypeptide (EDOT-BTDA-Pala), derived from L-alanine N-carboxyanhydride (L-Ala-NCA) via ring-opening polymerization using 4,7-bis(2,3-dihydrothieno[3,4-b][1,4]dioxin-5-yl)benzo[c][1,2,5]thiadiazole-5,6-diamine (EDOT-NH2-BTDA) as initiator, was employed as a glassy carbon electrode (GCE) covering host, in order to immobilize the METH-selective antibody. Prior to the examination of analytical features, GCE/EDOT-BTDA-Pala/Antibody surface was successfully characterized in the way of electrochemical (cyclic voltammetry and electrochemical impedance spectroscopy) and microscopic techniques (scanning electron microscopy and fluorescence microscopy). As for the analytical characterization, linearity and limit of detection (LOD) were found as 10–100 µg/mL with an equation of y=0.0429x-0.2347, (R2=0.996) and 13.07 µg/mL, respectively. Moreover, sample application using artificial urine, saliva and serum samples spiked with METH (10, 25, 50 µg/mL) were performed and LC-MS/MS system was used for further confirmation. The described platform can be adapted to monitor the other types of abused drugs by using suitably selected biorecognition elements. © 2016 Elsevier B.V.Ministry of Agriculture and Rural Development: 2016K121190 Ministry of Science, Technology and Space: STZ.2014 British Association for Psychopharmacology: 16-FEN-004This work was supported by Industrial Thesis Support Program (SAN-TEZ) of Republic of Turkey, Ministry of Science, Industry and Technology (Project Grant No: 0620.STZ.2014 ) and Republic of Turkey, Ministry of Development (Project Grant No: 2016K121190 ). This work was also partially supported by Ege University Research Fund through BAP project (Project No: 16-FEN-004 ) and Istanbul Technical University Research Fund. G. Bozokalfa from Ege University is acknowledged for the technical helps. Likrom Company was acknowledged for their support to the chromatographic analyses. One of the authors (I.C) thanks to TUBITAK for the financial support by means of “Fellowships For Visiting Scientists and Scientists On Sabbatical Leave” programme. Appendix A -