In vitro evaluation of Tc-99m- EDDA/tricine-HYNIC-Q-Litorin in gastrin-releasing peptide receptor positive tumor cell lines

WOS: 000318356800007PubMed ID: 23301856Bombesin and its derivatives exhibit a high affinity for gastrin-releasing peptide receptor (GRPr), which is over-expressed in a variety of human cancers (prostate, pancreatic, lung, etc.). The aim of this study was to investigate the in vitro potential of the hydrazinonicotinamide (HYNIC)-Q-Litorin. Tc-99m labeling was performed by using different co-ligands: tricine and ethylenediamine diacetic acid (EDDA). The radiochemical stability of radiolabeled peptide conjugates was checked at room temperature and in cysteine solution up to 24 h. The in vitro cell uptake of Tc-99m-EDDA-HYNIC-Q-Litorin and Tc-99m-tricine-HYNIC-Q-Litorin were evaluated on pancreatic tumor and control cell lines. Optimum specific activity and incubation time were determined for all the cell lines. The results showed that the cell uptake of the radiolabeled peptide conjugates in tumor cell lines were higher than in the control cell line. The findings of this study indicated the need for further development of in vivo study as a radiopharmaceutical for pancreatic tumor imaging.Scientific and Technological Research Council of TurkeyTurkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK); Scientific Project [108S200]; Ege University Science-Technology Application and Research CenterEge University [2009 BIL 025]The authors gratefully acknowledge the financial support received from the Scientific and Technological Research Council of Turkey, Scientific Project (108S200) and Ege University Science-Technology Application and Research Center (2009 BIL 025). The authors have declared no conflict of interest