Proteolytic Mediators in Gestational Diabetes Mellitus and Gingivitis

WOS: 000394511800010PubMed ID: 27762733Background: This study evaluates levels of matrix metalloproteinase (MMP)-8, MMP-9, and tissue inhibitor of MP-1 (TIMP-1) in biofluids of women with gestational diabetes mellitus (GDM) and systemically healthy counterparts with different statuses of periodontal health. Methods: Seventy-one women with GDM and gingivitis (Gg), 30 women with GDM and healthy periodontium (Gh), 28 systemically and periodontally healthy women (Hh), and 37 systemically healthy women with gingivitis (Hg) were evaluated. MMP-8, MMP-9, and TIMP-1 levels were determined in gingival crevicular fluid (GCF), saliva, and serum by immunofluorometric and enzyme-linked immunosorbent assays. Fullmouth clinical periodontal parameters were recorded. Results: GCF and serum MMP-8 concentrations, serum MMP-9 concentrations, and serum MMP-8/MMP-1 and MMP9/MMP-1 molar ratios were significantly higher in Gg compared with Hg group (P < 0.05). Serum MMP-8 levels and salivary TIMP-1 levels were higher in Gh compared with Hg group (P < 0.05) whereas salivary MMP-8/TIMP-1 molar ratio was lower in Gh compared with Hg group (P < 0.05). Elevated concentrations of GCF MMP-8 and MMP-9 were found in Gg compared with Gh group (P < 0.05). Significant correlations were found between local levels of biomarkers and clinical periodontal parameters in only GDM group. Conclusion: GDM may modulate both local and circulating levels of MMP-8 especially when associated with gingivitis.Ege University Research Foundation, Izmir, TurkeyEge University [2013-DIS-026]This study was supported by a grant from the Ege University Research Foundation, Izmir, Turkey (to: Ozgun Ozcaka, Pinar Gumus, Aliye Akcali, and Nurcan Buduneli) (2013-DIS-026). Dr. Timo Sorsa is an inventor of oral diagnostic United States patents (5652247, 57336341, 5866432, 6143476). The authors report no conflicts of interest related to this study