Microbial transformation of cycloastragenol

WOS: 000317452700013PubMed ID: 23357596The microbial transformation of cycloastragenol by the fungi Cunninghamella blakesleeana NRRL 1369 and Glomerella fusarioides ATCC 9552, and the bacterium Mycobacterium sp. NRRL 3805 were investigated. Both fungi mainly provided hydroxylated metabolites together with products formed by cyclization, dehydrogenation and Baeyer-Villiger oxidation resulting in a ring cleavage. The bacteria yielded only a single oxidation product, namely, 3-oxo-cycloastragenol. Structures of the metabolites were elucidated by 1-D (H-1,C-13), 2-D NMR (COSY, HMBC, HMQC) and HRMS analyses. (C) 2013 Elsevier Ltd. All rights regerved.TUBITAKTurkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [109S345]; COST Action CM [0804]This work is supported by TUBITAK (109S345) and COST Action CM 0804. We are very grateful to Dr. Markus Ganzera for his help in mass spectrometry, and Bionorm Natural Products for providing cycloastragenol