Synthesis and application of poly-SNS-anchored carboxylic acid: a novel functional matrix for biomolecule conjugation

WOS: 000293694700027Here we report the synthesis of a novel conducting polymer and its properties as an immobilization platform for biosensor application. The conducting polymer has functional groups used for the formation of amide bonding with the enzyme immobilized on the polymer surface. After covalent immobilization of glucose oxidase (GOx) on the polymeric matrix, its application for glucose biosensing was investigated in detail. Scanning electron microscopy (SEM), X-ray photoelectron spectroscopy (XPS) and contact angle measurements were used to monitor the surface properties of the polymer before and after biomolecule conjugation. The optimized biosensor showed a very good linearity between 0.01 mM and 1.2 mM, a 13 s response time and a detection limit (LOD) of 0.004 mM to glucose. Also, kinetic parameters, operational and storage stabilities were determined. Apparent Michaelis constant (K-m(app)) and I-max values of 1.17 mM and 11.28 mu A, respectively, were obtained.TUBATurkish Academy of Sciences; TUBITAKTurkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [110T580]This work is partially supported by TUBA and TUBITAK 110T580 grants. METU Central Laboratory is acknowledged for the SEM images. Merve Yuksel is gratefully acknowledged for her contributions in HPLC applications. Dr Selda Keskin is also gratefully acknowledged for access to the XPS facilities