Sensivity against some fungicides and molecular characterization of Botrytis cinerea isolates on grapes in Aegean region

3rd International Symposium on Postharvest Pathology - Using Science to Increase Food Availability -- JUN 07-11, 2015 -- Bari, ITALYWOS: 000392630500041Botrytis cinerea causing gray mold, is the most destructive pathogen on grapes. The intensive use of fungicides to control the disease leads to the acquisition of resistance by the pathogen and increased residues on grapes. In this study, B. cinerea isolates, collected from the vineyards in the Aegean Region, were tested for in vitro and in vivo sensitivity to the fungicides pyrimethanil, iprodione, fenhexamid, cyprodinil+fludioxonil, and boscalid. According to EDso values, the fungicides pyrimethanil and iprodione showed low effectiveness in reducing mycelial growth of isolates, while fenhexamid and cyprodinil+fludioxonil were highly effective. It was observed that at 5-10 mu g mL(-1), pyrimethanil and iprodione did not inhibit mycelia growth of 50 and 70% of B. cinerea isolates, respectively. Cyprodinil+fludioxonil mixture was the most effective in inhibiting mycelial growth of isolates, both in vitro and in vivo conditions. Cyprodinil+fludioxonil at dose of 3 lig mL-1 inhibited spore germination of all isolates of the pathogen. All isolates had a point mutation (G143A), which was detected with allel-specific primers BcAR-F and BcAR-R. Seven B. cinerea isolates were grouped in transposa. The presence of flipper element was not detected using F300 and F1500 primers.Int Soc Hort Sc