Optimization of Headspace-Solid Phase Microextraction (HS-SPME) technique for the analysis of volatile compounds of margarine

The objective of the study was to develop optimum Headspace-Solid Phase Microextraction (HS-SPME) conditions for determining volatile compounds of margarine. The effects of fiber type, extraction temperature, extraction time on extraction rates of 2,3 butandione, dimethyl disulfide, nonanal, butanoic acid, hexanoic acid, octanoic acid, ?-nonalactone, ?-decalactone, total area and number of volatile compounds were investigated. A response surface methodology was applied using three independent variables such as fiber type, extraction temperature and extraction time. The fiber type, extraction temperature and extraction time were arranged with Box–Behnken design. The results shows that extraction temperature and extraction time affect both total area and number of volatile compounds but fiber type effect total area of volatile compounds. The optimum HS-SPME conditions were determined as 47.54 °C extraction temperature and 33.63 min extraction time using DVB/CAR/PDMS fiber type. © 2019, Association of Food Scientists & Technologists (India).Firat University Scientific Research Projects Management Unit, FÃ?BAP: 16/MUH/028This study was supported by Ege University Scientific Research Projects Coordination Unit (Project No: 16/MUH/028). The authors would also like to thank Aromsa, Inc. for supplying the aroma used in this research. Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. -