Single-step purification and characterization of an extreme halophilic, ethanol tolerant and acidophilic xylanase from Aureobasidium pullulans NRRL Y-2311-1 with application potential in the food industry

WOS: 000389909100010PubMed ID: 27979257An extracellular xylanase from Aureobasidium pullulans NRRL Y-2311-1 produced on wheat bran was purified by a single-step chromatographic procedure. The enzyme had a molecular weight of 21.6 kDa. The optimum pH and temperature for xylanase activity were 4.0 and 30-50 degrees C, respectively. The enzyme was stable in the pH range of 3.0-8.0. The inactivation energy of the enzyme was calculated as 218 kJ mol(-1). The xylanase was ethanol tolerant and kept complete activity in the presence of 10% ethanol. Likewise, it retained almost complete activity at a concentration range of 0-20% NaCl. In general, the enzyme was resistant to several metal ions and reagents. Mg2+, Zn2+, Cu2+, K1+, EDTA and beta-mercaptoethanol resulted in enhanced xylanase activity. The K-m and V-max values on beechwood xylan were determined to be 19.43 mg ml(-1) and 848.4 U ml(-1), respectively. The enzyme exhibits excellent characteristics and could, therefore, be a promising candidate for application in food and bio-industries. (C) 2016 Elsevier Ltd. All rights reserved.Scientific and Technological Research Council of Turkey - TUBITAKTurkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [TOVAG 112O521]; Ege University Science and Technology Center - EBILTEMEge University [2013/BIL/025]This work was financially supported by The Scientific and Technological Research Council of Turkey - TUBITAK (Project No: TOVAG 112O521) and Ege University Science and Technology Center - EBILTEM (Project No: 2013/BIL/025). This work is also included in Cost Action TD1203 entitled as "Food Waste Valorization for Sustainable Chemicals, Materials and Fuels (EUBis)" and Cost Action FP1306 entitled as "Valorisation of Lignocellulosic Biomass Side Streams for Sustainable Production of Chemicals, Materials & Fuels Using Low Environmental Impact Technologies"