TA-cloning vectors for rapid and cheap cloning of zebrafish transgenesis constructs

Here we report the generation and use of three multisite Gateway TA-cloning plasmids, p5E-TA, pME-TA, and p3E-TA, to enable rapid and cheap construction of new entry vectors and final Tol2-transgenesis vectors for zebra fish transgenesis, as well as a TA-cloning expression vector, pCS2 + TA, for rapid generation of new expression plasmids