Dianthus erinaceus var. erinaceus: Extraction, isolation, characterization and antimicrobial activity investigation of novel saponins

WOS: 000378027300039A phytochemical analysis of Dianthus erinaceus Boiss. var. erinaceus (Caryophyllaceae) has led to the isolation of two novel triterpenoid saponins, containing an oleane type skeleton, named dianosides K and L (1, 2), along with six known triterpenoid saponins (3-8). On the basis of chemical and spectrometric data, the structures of the new compounds were elucidated as 3-O-[beta-D-glucopyranosyl (1 -> 3)]-[beta-D-glucopyranosyl (1 -> 6)]-beta-D-glucopyranosyl-olean-12-ene-23 alpha,28-beta-dioic acid 28-O-beta-D-glucopyranosyl ester (1) and 3-O-[beta-D-glucopyranosyl (1 -> 3)]-[beta-D-glucopyranosyl(1 -> 6)]-beta-D-glucopyranosyl-olean-12-ene-23 alpha,28-beta-dioic acid 28-O-alpha-L-mannopyranosyl (1 -> 6)-beta-D-glucopyranosyl ester (2). All isolated natural compounds were structurally characterized by 1D-(H-1, C-13, DEPT); 2D-(COSY, HMQC, HMBC) NMR and HR-ESI/MS methods. The antimicrobial activity of compounds 1 and 2 were tested against four Gram-negative, three Gram-positive bacteria and the yeast Candida albicans by the MIC method. (C) 2016 Phytochemical Society of Europe. Published by Elsevier B.V. All rights reserved.Research Grant Office of Ege University Izmir-TurkeyEge University [2012 FEN 042]The authors are grateful to Research Grant Office of Ege University Izmir-Turkey (2012 FEN 042) for financial support. We are thankful to Assoc. Prof. Dr. Levent SIK for collection and identification of the plant and Enago Crimson Interactive Inc. (USA) for proofreading. We would like to thank TUBITAK (Marmara Research Center, Gebze, Kocaeli) for running NMR (600 MHz) and HR-ESI/MS spectra and Ege University (EBILTEM) for running NMR (400 MHz) spectra