Cells-on-nanofibers: Effect of polyethyleneimine on hydrophobicity of poly-epsilon-caprolacton electrospun nanofibers and immobilization of bacteria

WOS: 000466821100004PubMed ID: 31000161Among other synthetic polymers, poly-E-caprolacton (PCL) nanofibers are one of the most popular ones, especially in tissue engineering application due to its distinct mechanical and chemical properties. However, in some cases, lacking functional group on polymer structure obstructs the covalent modification of the PCL nanofibers for the aim. Herein, polyethyleneimine (PEI) was blended with PCL polymer to provide functional amino groups on the surface of the nanofiber mat. PCL-PEI nanofiber was successfully constructed and preparation parameters were optimized. Scanning electron microscopy (SEM) and contact angle measurements were carried out to characterize the PCL-PEI nanofiber. After characterization, Gluconobacter oxydans was immobilized on the surface by the help of glutaraldehyde chemistry. Cyclic voltammetry (CV) and differential pulse voltammetry (DPV) measurements were carried out to prove the success of surface modification. In addition, scanning electron microscopy images were also taken after the immobilization of G. oxydans on PCL-PEI nanofiber mat. For the first time in this study, one microorganism was immobilized onto the electrospun nanofiber mat by covalent modification. In conclusion, PCL-PEI/G. oxydans whole-cell biosensor was tested for sensing of glucose as a model analyte.Scientific and Technological Research Council of Turkey (TUBITAK)Turkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [215Z194]; Aliye Uster Foundation; Turkish Academy of Sciences The Young Scientists Award Programme (TUBA-GEBIP2015)Authors acknowledge The Scientific and Technological Research Council of Turkey (TUBITAK; Project 215Z194) and Aliye Uster Foundation for funding this research. M. Gordegir, S. Oz and M. Buhur thank to TUBITAK 2209 A-Program for the University Students at Undergraduate Level. Dr. D. O Demirkol acknowledges the support from the Turkish Academy of Sciences The Young Scientists Award Programme (TUBA-GEBIP2015)