A New Approach for Bating: Reactive Red 120 Dye Attached Poly (Hema) Nanoparticles

###EgeUn###In the present work, Reactive Red 120-attached poly (2-hydroxy ethyl methacrylate) nanoparticles (PHEMA-RR120) were synthesized as an adsorbent for the removal of the proteins albumin and globulin. PHEMA nanoparticles were first prepared by surfactant-free emulsion polymerisation. The dye ligand Reactive Red 120 was chemically attached to the PHEMA nanoparticles via a nucleophilic substitution reaction. Characterisation of the PHEMA-RR120 nanoparticles was carried out by specific surface area, dry mass weight of the nanoparticle, SEM, FTIR and Zeta Size analysis. Albumin and globulin adsorption of PHEMA-RR120 nanoparticles was investigated with respect to process conditions (temperature, pH, time, initial concentration and ionic strength), and the re-usability of PHEMA-RR120 was also determined. The albumin and globulin protein adsorption of PHEMA-RR120 nanoparticles was found to be respectively 24.60g/g and 3.95g/g at 25 degrees C, 12.58g/g and 3.86g/g at 37 degrees C, 11.45g/g and 2.69g/g at 45 degrees C. The results show that PHEMA-RR120 nanoparticles can be used for the effective, reusable removal of albumin and globulin due to their large surface area and high adsorption capacity.Ege University Scientific Research Projects Coordination Unit for Financial Support [13MUH009]This study was financially supported by Ege University Scientific Research Projects Coordination Unit for Financial Support (Project Number: 13MUH009)