Voltammetric and impedimetric detection of DNA hybridization by using dendrimer modified graphite electrodes

WOS: 000336115900015In the present study, the electrochemical sensor was designed by the modification of the fourth generation poly(amido amine) (PAMAM) dendrimer onto the surface of pencil graphite electrode (G4-PDR/PGE) and this single-use dendrimer modified electrode was then examined for advanced nucleic acid detection by using electrochemical techniques. After the covalent immobilization of G4-PDR onto the surface of carboxylated PGE surface, the surface characterization of dendrimer modified PGE was investigated by using scanning electron microscopy (SEM), and also, different electrochemical techniques: cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). The voltammetric monitoring of a sequence-selective DNA hybridization related to Hepatitis B virus (HBV) sequences was accordingly performed in the presence of the complementary of probe (target), and also the mixture medium containing target:non-complementary (NC) sequences (1:1). (C) 2014 Elsevier B.V. All rights reserved.Turkish Scientific and Technological Research Council (TUBITAK)Turkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [111T073]; Ege University Scientific Research Project CoordinationEge University [13/ECZ/005]; Turkish Academy of Sciences (TUBA)Turkish Academy of Sciences; TUBITAKTurkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [111T073]A.E acknowledges the financial support from Turkish Scientific and Technological Research Council (TUBITAK; Project No. 111T073), Ege University Scientific Research Project Coordination (Project No. 13/ECZ/005), and she also would like to express her gratitude to the Turkish Academy of Sciences (TUBA) as an Associate member for its partial support. F.M. and G.C. acknowledge a master project scholarship through by TUBITAK (Project No.111T073)