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Not AvailableThe bacterial extracellular polymeric substances (EPS) have huge applications in biotechnological and industrial sector. Biochemical composition determines its role in biofilm formation and pathogenicity as well as beneficial applications in various industries as thickeners, stabilizers, gelling agents etc. In the present study, aimed at screening the EPS producing bacteria from three species of brown seaweed and the EPS producing bacteria was isolated from Sargassium wightii. The EPS extraction was optimized and maximum production was recorded in BHI broth and media supplemented with glucose. The extracted EPS contained 69.9% carbohydrate. Structural analysis by FTIR revealed the presence of carbohydrate and S-S stretch. The isolate was identified as Bacillus cereus by 16S rRNA sequencing and species specific PCR targeting bla gene. Complete study on biochemical composition and structure of EPS will facilitate the employment of this novel biopolymer in food and pharmaceutical industry.Not Availabl