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Not AvailableBackground: Surra is caused by Trypanosoma evansi and is considered as one of the important disease in camels in India. Very often camel acts as the carrier of the infection with very low level of parasitaemia which cannot be detected by conventional blood smear examination. Methodology: In the present study, a pilot sero-epidemiological study was conducted among camels for trypanosomosis in Rajasthan using purified flagellar (FLA) antigen based indirect enzyme linked immunosorbent assay (I-ELISA) and compared with standard CATT/ T. evansi test. The diagnostic potentiality of purified FLA antigen based I-ELISA (FLA-I-ELISA) were evaluated using 230 camel sera samples from different districts of Rajasthan including Jaipur, Udaipur, Bikaner, Jodhpur, and Ajmer. Results: The test showed an overall 23. 04% sero positivity (S. P). In Jaipur, S. P was found to be 23. 07%, in Udaipur 25. 5%, in Bikaner 18. 18%, in Jodhpur 25. 3% and in Ajmer, S. P was found to be 23. 33%. Conclusion: Thus, epidemiological survey were done in camels with purified FLA-I-ELISA test in different districts of Rajasthan and revealed that, Udaipur showed the maximum sero positivity for trypanosomosis.Not Availabl