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Not AvailableCephalopod is an important seafood variety exported from Gujarat, which contributes 8.81 per cent of total marine landing in Gujarat. In cephalopods, cuttlefish are exported as frozen block or IQF product. The present work was intended to study the effect of commercial additive on the quality of cuttlefish under refrigerated storage condition. Samples were segregated into four lots viz. treated with salt, with commercial additive hidramor-4, with salt and additive and the untreated to serve as control. The samples were frozen in Individual Quick Freezer consequently packed in multilayer film of EVOH pouches and held in refrigerated for further study. Samples were periodically analysed for the changes in sensory, biochemical and microbiological quality characteristics on day 1, 3, 6 and 9 under refrigerated storage condition. The initial TVBN and TMA values were 2.7- 3.0 mg per 100 gram and 1.7-2.0 mg per 100 gram respectively, and it increased progressively in all the samples with the time of storage. Total plate count, Enterobacteriaceae, H2S producing bacteria and pseudomonas sp were analysed during storage. The initial count of Enterobacteriaceae, H2S producing bacteria and pseudomonas sp. were 3.4-4 log for treated samples. This study revealed that additive increased the weight gain, tenderness compared to salt and untreated samples and it not influence in the shelf life during refrigerated storage.Not Availabl