ch PRL –siRNA IGF1 anterior pituitary

Not AvailableUp-regulation of the chicken prolactin (chPRL) has been implicated in incubation behavior in galliforms that induces broodiness and gonadal involution. In this study, we evaluated the knock down of PRL in hen anterior pituicytes cultured in vitro by small-interfering RNA (siRNA) targeting of chPRL, as well as the efficacy of this treatment on PRL gene expression, prolactin receptor (PRLR) and insulin like growth factor I (IGF-I).The chPRLtargeted siRNA were chemically synthesized and constructed based on chicken and turkey PRL mRNA. Expression of chPRL was analyzed by polymerase chain reaction (PCR) with designed primers. Culture media were assayed for IGF-I content utilizing the immuno radio metric assay (IRMA) Kit. Results indicate that, significant (P