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Not AvailableTo determine the buffalo breed difference in methane emission, six animals three each of lactating Bhadari and Murrah buffalo with mean body weight of 441.87±7.95 kg and 515.40±2.54 kg were used to estimate the nutrients digestibility and methane production. Animals of both breeds were fed wheat straw-concentrate for one month and a digestion trial was conducted for 6 days. During this period gas expired by animals was collected from each animal in canisters for 24 h following SF6 technique. Gas samples (4 to 5) were collected from each animal to estimate the CH4 production. Dry matter intake of Murrah bufflo (12.26) was significantly (P<0.05) higher than Bhadawari (8.96 kg/d). The dry matter and organic matter digestibility of wheat straw-concentrate diet was similar between both Bhadawari and Murrah buffalo breeds. Crude protein digestibility tended to be higher in Bhadawari (62.27) than Murrah buffalo (57.49%). The ADF and cellulose digestibility was relatively less in Bhadawari (41.45 and 58.81) than Murrah buffaloes (45.67 and 62.44%), while NDF and hemi-cellulose digestibility was at par between both buffalo breeds. Methane production g/kg DDMI was lower (P<0.05) in Bhadwari (21.49 and 34.96) than Murrah breed buffaloes (23.26 and 41.88) on wheat straw-concentrate diet. Methane production (g/kg milk) was lower (P