Not AvailableThe aim of this article is to express envelope glycoprotein E2 of bovine viral diarrhea virus (BVDV) in yeast Pichia pastoris and its utility as a diagnostic antigen in ELISA. The BVDV E2 gene was cloned into the pPICZαA vector followed by integration into the Pichia pastoris strain X-33 genome for methanol-induced expression. SDS-PAGE and Western blot results showed that the recombinant BVDV E2 protein (72 kDa) was expressed and secreted into the medium at a concentration of 40 mg/L of culture under optimized conditions. An indirect ELISA was then developed by using the yeast-expressed E2 protein. Preliminary testing of 300 field cattle serum samples showed that the E2 ELISA showed a sensitivity of 91.07% and a specificity of ...
Background: The biological characteristics of BoHV-4 make it a good candidate as a gene delivery vec...
A region of genome from the NADL strain of BVDV corresponding to the coding sequence for the E2 glyc...
AbstractBovine viral diarrhea virus (BVDV) is an important cause of economic losses worldwide. E2 is...
Not AvailableThe aim of this study was to develop an indirect ELISA using the helicase domain of bov...
which permits unrestricted use, distribution, and reproduction in any medium, provided the original ...
Bovine viral diarrhea virus (BVDV) is a RNA virus and a prototype member of the pestivirus genus in ...
The BVDV envelope glycoprotein Erns/gp48 and the C terminal 79 amino acids of the capsid protein cod...
RIGHTS : This article is licensed under the BioMed Central licence at http://www.biomedcentral.com/...
Bovine Viral Diarrhea Virus (BVDV) is the causative agent of a worldwide disease. The virus infects ...
SIGLEAvailable from British Library Document Supply Centre-DSC:DXN011311 / BLDSC - British Library D...
Funding: This work was supported by the Taiwan Ministry of Science and Technology [MOST-106-2911-I-0...
Bovine viral diarrhea-mucosal disease (BVD-MD) is caused by bovine viral diarrhea virus (BVDV), and ...
The putative gene encoding the cytopathic bovine viral diarrhoea virus (BVDV) Osloss strain p80 prot...
Bovine viral diarrhea (BVO), caused by the BVO virus, has been recognized in many parts of the world...
This article describes the expression of a truncated form of bovine herpesvirus 1 (BoHV-1) glycoprot...
Background: The biological characteristics of BoHV-4 make it a good candidate as a gene delivery vec...
A region of genome from the NADL strain of BVDV corresponding to the coding sequence for the E2 glyc...
AbstractBovine viral diarrhea virus (BVDV) is an important cause of economic losses worldwide. E2 is...
Not AvailableThe aim of this study was to develop an indirect ELISA using the helicase domain of bov...
which permits unrestricted use, distribution, and reproduction in any medium, provided the original ...
Bovine viral diarrhea virus (BVDV) is a RNA virus and a prototype member of the pestivirus genus in ...
The BVDV envelope glycoprotein Erns/gp48 and the C terminal 79 amino acids of the capsid protein cod...
RIGHTS : This article is licensed under the BioMed Central licence at http://www.biomedcentral.com/...
Bovine Viral Diarrhea Virus (BVDV) is the causative agent of a worldwide disease. The virus infects ...
SIGLEAvailable from British Library Document Supply Centre-DSC:DXN011311 / BLDSC - British Library D...
Funding: This work was supported by the Taiwan Ministry of Science and Technology [MOST-106-2911-I-0...
Bovine viral diarrhea-mucosal disease (BVD-MD) is caused by bovine viral diarrhea virus (BVDV), and ...
The putative gene encoding the cytopathic bovine viral diarrhoea virus (BVDV) Osloss strain p80 prot...
Bovine viral diarrhea (BVO), caused by the BVO virus, has been recognized in many parts of the world...
This article describes the expression of a truncated form of bovine herpesvirus 1 (BoHV-1) glycoprot...
Background: The biological characteristics of BoHV-4 make it a good candidate as a gene delivery vec...
A region of genome from the NADL strain of BVDV corresponding to the coding sequence for the E2 glyc...
AbstractBovine viral diarrhea virus (BVDV) is an important cause of economic losses worldwide. E2 is...