The use of Digital Game-Based Learning (DGBL) in manufacturing education has been on the rise. However, empirical evidence of the educational effectiveness of DGBL is still scant, especially in manufacturing education. This paper reports on the development of a Life Cycle Assessment (LCA) game used in the teaching of sustainable manufacturing at tertiary level by having students apply their knowledge. The development of the LCA Game covered a period of three years, including testing and co-design activities involving in total 265 students. The final version of the LCA Game was tested with a class of 33 master students of management engineering by means of a quasi-experimental with pre-test and post-test design. The results show a statistica...