Recombinant production of complex eukaryotic proteins for structural analyses typically requires a profound screening process to identify suitable constructs for the expression of ample amounts of properly folded protein. Furthermore, the evaluation of an optimal expression host has a major impact on protein yield and quality as well as on actual cost of the production process. Here we present a novel fast expression system for multiple hosts based on a single donor vector termed pFlp-Bac-to-Mam. The range of applications of pFlp-Bac-to-Mam comprises highly efficient transient transfection of HEK293-6E in serum-free suspension culture and subsequent large-scale production of challenging proteins expressing in mg per Liter level using either...
International audienceAbstract The Baculovirus/insect cell expression system is a powerful technolog...
The baculovirus system is an extremely powerful tool for expression of heterologous genes in eukaryo...
Mammalian expression systems have an undisputed long-standing and very successful history for the ge...
Recombinant production of complex eukaryotic proteins for structural analyses typically requires a p...
The increasing demand for production and characterization of diverse groups of recombinant proteins ...
The High Five (H5) cell line, derived from the lepidopteran Trichoplusia ni, is one of the major ins...
There is increasing demand for high quality, biologically active recombinant proteins in awide range...
A prerequisite for structural and biochemical analyses of proteins is the availability of large amou...
Baculovirus-insect cell expression system has become one of the most widely used eukaryotic expressi...
Since the number of potential drug targets identified has significantly increased in the past decade...
Recent advances in genomics, proteomics, and structural biology raised the general need for signific...
Many research projects in life sciences require purified biologically active recombinant protein. In...
Baculovirus-insect cell expression system has become one of the most widely used eukaryotic expressi...
Baculovirus-insect cell expression system has become one of the most widely used eukaryotic expressi...
Structural, biochemical and biophysical studies of eukaryotic soluble and membrane proteins require ...
International audienceAbstract The Baculovirus/insect cell expression system is a powerful technolog...
The baculovirus system is an extremely powerful tool for expression of heterologous genes in eukaryo...
Mammalian expression systems have an undisputed long-standing and very successful history for the ge...
Recombinant production of complex eukaryotic proteins for structural analyses typically requires a p...
The increasing demand for production and characterization of diverse groups of recombinant proteins ...
The High Five (H5) cell line, derived from the lepidopteran Trichoplusia ni, is one of the major ins...
There is increasing demand for high quality, biologically active recombinant proteins in awide range...
A prerequisite for structural and biochemical analyses of proteins is the availability of large amou...
Baculovirus-insect cell expression system has become one of the most widely used eukaryotic expressi...
Since the number of potential drug targets identified has significantly increased in the past decade...
Recent advances in genomics, proteomics, and structural biology raised the general need for signific...
Many research projects in life sciences require purified biologically active recombinant protein. In...
Baculovirus-insect cell expression system has become one of the most widely used eukaryotic expressi...
Baculovirus-insect cell expression system has become one of the most widely used eukaryotic expressi...
Structural, biochemical and biophysical studies of eukaryotic soluble and membrane proteins require ...
International audienceAbstract The Baculovirus/insect cell expression system is a powerful technolog...
The baculovirus system is an extremely powerful tool for expression of heterologous genes in eukaryo...
Mammalian expression systems have an undisputed long-standing and very successful history for the ge...