Characteristics of Indigenous Probiotic from River Buffalo Milk in North Sumatera Indonesia

The research aimed to know the probiotic characteristics of Lactic Acid Bacteria (LAB) was isolated from river buffalo milk in North Sumatera Indonesia. A total of 41 LAB isolated from river buffalo milk, were tested for bile salt condition, antimicrobial activity and ability to attach on intestinal mucosa. Result showed all of the isolates were able to survive in the presence of 0.5% bile salt and there were decreasing viability cell number from 1.1 to 3.3 log cfu/ml. The ten isolates have antibacterial effect on the indicator microorganisms and were able to attach on intestinal mucosa. An increasing of LAB cell numbers attach on intestinal mucosa ranged from 0.7 to 1.7 log cfu/cm2. In conclusion, ten isolates from river buffalo milk have some probiotic properties and potential as a candidate probiotic bacterium