Efficiency and precision of microRNA biogenesis modes in plants
- Moro, Belén
- Chorostecki, Uciel Pablo
- Arikit, Siwaret
- Suarez, Irina Paula
- Hobartner, Claudia
- Rasia, Rodolfo Maximiliano
- Meyers, Blake C.
- Palatnik, Javier Fernando
DOIAbstract
Many evolutionarily conserved microRNAs (miRNAs) in plants regulate transcription factors with key functions in development. Hence, mutations in the core components of the miRNA biogenesis machinery cause strong growth defects. An essential aspect of miRNA biogenesis is the precise excision of the small RNA from its precursor. In plants, miRNA precursors are largely variable in size and shape and can be processed by different modes. Here, we optimized an approach to detect processing intermediates during miRNA biogenesis. We characterized a miRNA whose processing is triggered by a terminal branched loop. Plant miRNA processing can be initiated by internal bubbles, small terminal loops or branched loops followed by dsRNA segments of 15-17 bp...
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