CLCA2 epigenetic regulation by CTBP1, HDACs, ZEB1, EP300 and miR-196b-5p impacts prostate cancer cell adhesion and EMT in metabolic syndrome disease
- Porretti, Juliana Carla
- Dalton, Guillermo Nicolás
- Massillo, Cintia Lorena
- Scalise, Georgina Daniela
- Farré, Paula Lucía
- Elble, Randolph
- Gerez, Esther Noemi
- Accialini, Paula Lucia
- Cabanillas, Ana Maria de Los A.
- Gardner, Kevin
- de Luca, Paola
- de Siervi, Adriana
DOIAbstract
Prostate cancer (PCa) is the most common cancer among men. Metabolic syndrome (MeS) is associated with increased PCa aggressiveness and recurrence. Previously, we proposed C-terminal binding protein 1 (CTBP1), a transcriptional co-repressor, as a molecular link between these two conditions. Notably, CTBP1 depletion decreased PCa growth in MeS mice. The aim of this study was to investigate the molecular mechanisms that explain the link between MeS and PCa mediated by CTBP1. We found that CTBP1 repressed chloride channel accessory 2 (CLCA2) expression in prostate xenografts developed in MeS animals. CTBP1 bound to CLCA2 promoter and repressed its transcription and promoter activity in PCa cell lines. Furthermore, we found that CTBP1 formed a ...
Add to ORKG