Monitoring biofilm formation by using cyclic voltammetry – effect of the experimental conditions on biofilm removal and activity

The effect of experimental conditions on cyclic voltammetry experiments on platinum electrodescovered with biofilms formed by Pseudomonas fluorescens for 2 hours was investigated. Results show thatrecycling the potential stabilizes the shape of the cyclic voltammogram after 135 cycles, but the observationof the electrodes by epifluorescence microscopy showed that cells are still adhered to the platinum surface.Some experimental conditions were changed during the electrochemical measurements – sweep rate, pH ofthe buffer and applied potential range. Some of these parameters had a strong impact on the bacteria thatare adhered to the surface, increasing the death and removal in some circumstances