Add to ORKGIt is widely believed that discovery of specific, sensitive and reliable tumor biomarkers can improve the treatment of cancer. The goal of this study was to develop a novel fractionation protocol targeting hydrophobic proteins as possible cancer cell membrane biomarkers. Hydrophobic proteins of breast cancer tissues and cell lines were enriched by polymeric reverse phase columns. The retained proteins were eluted and digested for peptide identification by nano-liquid chromatography with tandem mass spectrometry using a hybrid linear ion-trap Orbitrap. Hundreds of proteins were identified from each of these three specimens: tumors, normal breast tissue, and breast cancer cell lines. Many of the identified proteins defined key cellular functi...
BackgroundMany high-abundant acute phase reactants have been previously detected as potential breast...
textabstractBreast cancer is the most commonly diagnosed malignancy in women in the Western world, w...
This work develops a multidimensional liquid-phase separation protocol---Proteosep technique---for t...
It is widely believed that discovery of specific, sensitive, and reliable tumor biomarkers can impro...
The separation and detection of proteins and peptides are crucial in elucidating disease processes a...
There is substantial interest in applying proteomics to cancer biomarker discovery. Proteomics is pa...
BACKGROUND:Breast cancer is the fifth most prevalent cause of death among women worldwide. It is als...
Breast cancer is the most common gynecological cancer in developed nations and represents a signific...
The purpose of the studies included in this thesis is to develop an effective an efficient method to...
In this work, a two-dimensional liquid-phase separation technique coupled with time-of-flight mass s...
We have begun an early phase of biomarker discovery in three clinically important types of breast ca...
We have conducted proteome-wide analysis of fresh surgery specimens derived from breast cancer patie...
Separation and identification of proteins in proteomics have been important and challenging due to t...
Non-porous reversed-phase (NP-RP) HPLC has been used to rapidly generate protein profiles of whole c...
Background: Breast cancer is the most common cancer in women in most areas of the world. In Iran and...
BackgroundMany high-abundant acute phase reactants have been previously detected as potential breast...
textabstractBreast cancer is the most commonly diagnosed malignancy in women in the Western world, w...
This work develops a multidimensional liquid-phase separation protocol---Proteosep technique---for t...
It is widely believed that discovery of specific, sensitive, and reliable tumor biomarkers can impro...
The separation and detection of proteins and peptides are crucial in elucidating disease processes a...
There is substantial interest in applying proteomics to cancer biomarker discovery. Proteomics is pa...
BACKGROUND:Breast cancer is the fifth most prevalent cause of death among women worldwide. It is als...
Breast cancer is the most common gynecological cancer in developed nations and represents a signific...
The purpose of the studies included in this thesis is to develop an effective an efficient method to...
In this work, a two-dimensional liquid-phase separation technique coupled with time-of-flight mass s...
We have begun an early phase of biomarker discovery in three clinically important types of breast ca...
We have conducted proteome-wide analysis of fresh surgery specimens derived from breast cancer patie...
Separation and identification of proteins in proteomics have been important and challenging due to t...
Non-porous reversed-phase (NP-RP) HPLC has been used to rapidly generate protein profiles of whole c...
Background: Breast cancer is the most common cancer in women in most areas of the world. In Iran and...
BackgroundMany high-abundant acute phase reactants have been previously detected as potential breast...
textabstractBreast cancer is the most commonly diagnosed malignancy in women in the Western world, w...
This work develops a multidimensional liquid-phase separation protocol---Proteosep technique---for t...