Supplementary Figures 1 Supplementary Materials

Figure S1. Ligand tuning of larval olfactory neurons (A) Odor response profiles of 8 OSNs, measured at axon termini of a given OSN in the antennal lobe, against a panel of 22 odorants (10-2 dilution) and paraffin oil (solvent), are shown as described in Figure 1d. Traces from n=7-9 animals per stimulus are stacked. Two OSNs were routinely labeled with G-CaMP by crossing two homozygous strains carrying each of the 11 Gal4 drivers (including Or35a, Or42a, and Or42b) together with UAS-G-CaMP, allowing us to image simultaneously from two OSNs. Combinations were chosen to maximize separation of the two axon terminals of the labeled OSNs (Figure 1 b-c: see "Genotype of Drosophila strains used in this paper " for the complete list of genotypes used). (B) Summary of ligand specificity of ORs expressed in both larval and adult OSNs, from this study and from the literature [1]. No ligands have been found to activate the four receptors in gray (Or2a, Or33b [1]; Or33a, this study; Or49a, [2, 3]). (C) Summary of ligand specificity of larval-specific ORs from this study and previous studies [3, 4]. Calcium imaging carried out in this study showed that larval OSNs can b