Lensless holographic microscope of biological samples

Lensless holographic microscopy shows high potential to be a practical point of care diagnostic tool due to its unique features like compactness, simple prototype, portability and on-chip operation. In the present work, a deep violet (370nm, 10 nm FWHM) LED at low power is used as a low coherent light source for lensless 3-D holographic imaging of live cells and microparticles. An on-chip Fresnel hologram of the sample is recorded in on-axis geometry on a high resolution CMOS camera and 3-D phase reconstruction has been performed eliminating the twin images using an optimization approach