Deciphering rhizosphere microbiome assembly of wild and modern common bean (Phaseolus vulgaris) in native and agricultural soils from Colombia.

Abstract: Background: Modern crop varieties are typically cultivated in agriculturally well-managed soils far from the centers oforigin of their wild relatives. How this habitat expansion impacted plant microbiome assembly is not well understood. Results: Here, we investigated if the transition from a native to an agricultural soil affected rhizobacterial communityassembly of wild and modern common bean (Phaseolus vulgaris) and if this led to a depletion of rhizobacterialdiversity. The impact of the bean genotype on rhizobacterial assembly was more prominent in the agriculturalsoil than in the native soil. Although only 113 operational taxonomic units (OTUs) out of a total of 15,925 wereshared by all eight bean accessions grown in native and agricultural soils, this core microbiome represented a largefraction (25.9%) of all sequence reads. More OTUs were exclusively found in the rhizosphere of common bean in theagricultural soil as compared to the native soil and in the rhizosphere of modern bean accessions as compared to wildaccessions. Co-occurrence analyses further showed a reduction in complexity of the interactions in the beanrhizosphere microbiome in the agricultural soil as compared to the native soil