Genotoxic and Cytotoxic Responses of Recombinant Salmonella typhimurium TA1535 Induced by Simulated Space Radiation Qualities

Introduction: For risk assessment, the mutagenic potential of the heavy ion component of the galactic cosmic radiation is of major concern for tumor induction as radiation late effects. The genetically modified TA1535 Salmonella typhimurium strain (SWITCH) reports on the presence of genotoxic conditions by dose dependent increase of bioluminescence induction and on the presence of cytotoxic conditions by dose dependent decrease in GFP fluorescence. By this, it is possible to analyze bacterial inactivation and mutation induction by ionizing radiation in parallel in the same cell within short time