The identification of bona fide protein-protein interactions and the mapping of proteomes was greatly enhanced by protein tagging for generic affinity purification methods and analysis by mass spectrometry (AP-MS). The high quality of AP-MS data permitted the development of proteomic navigation by sequential tagging of identified interactions. However AP-MS is laborious and limited to relatively high affinity protein-protein interactions. Proximity labeling, first with the biotin ligase BirA, termed BioID, and then with ascorbate peroxidase, termed APEX, permits a greater reach into the proteome than AP-MS enabling both the identification of a wider field and weaker protein-protein interactions. This additional reach comes with the need for...
If one considers chemical-biology toolsets that have had the greatest impact on numerous fields of l...
If one considers chemical-biology toolsets that have had the greatest impact on numerous fields of l...
If one considers chemical-biology toolsets that have had the greatest impact on numerous fields of l...
Within cells, proteins can co-assemble into functionally integrated and spatially restricted multico...
Abstract Specialized biological processes occur in different regions and organelles of the cell. Add...
This is the final version of the article. It was first available from the American Society for Bioch...
Proximity labeling catalyzed by promiscuous enzymes, such as APEX2, has emerged as a powerful approa...
International audienceThe development of new approaches is critical to gain further insights into bi...
International audienceThe development of new approaches is critical to gain further insights into bi...
Thesis: Ph. D., Massachusetts Institute of Technology, Department of Chemistry, 2018.Cataloged from ...
Protein-protein interactions govern almost all cellular functions. These complex networks of stable ...
ABSTRACT: Accurate and sensitive detection of protein−protein and protein−RNA interactions is key to...
Compartmentalization is an essential characteristic of eukaryotic cells, ensuring that cellular proc...
Protein–protein interactions are at the core of all cellular functions and dynamic alterations in pr...
Protein-protein interactions govern almost all cellular functions. These complex networks of stable ...
If one considers chemical-biology toolsets that have had the greatest impact on numerous fields of l...
If one considers chemical-biology toolsets that have had the greatest impact on numerous fields of l...
If one considers chemical-biology toolsets that have had the greatest impact on numerous fields of l...
Within cells, proteins can co-assemble into functionally integrated and spatially restricted multico...
Abstract Specialized biological processes occur in different regions and organelles of the cell. Add...
This is the final version of the article. It was first available from the American Society for Bioch...
Proximity labeling catalyzed by promiscuous enzymes, such as APEX2, has emerged as a powerful approa...
International audienceThe development of new approaches is critical to gain further insights into bi...
International audienceThe development of new approaches is critical to gain further insights into bi...
Thesis: Ph. D., Massachusetts Institute of Technology, Department of Chemistry, 2018.Cataloged from ...
Protein-protein interactions govern almost all cellular functions. These complex networks of stable ...
ABSTRACT: Accurate and sensitive detection of protein−protein and protein−RNA interactions is key to...
Compartmentalization is an essential characteristic of eukaryotic cells, ensuring that cellular proc...
Protein–protein interactions are at the core of all cellular functions and dynamic alterations in pr...
Protein-protein interactions govern almost all cellular functions. These complex networks of stable ...
If one considers chemical-biology toolsets that have had the greatest impact on numerous fields of l...
If one considers chemical-biology toolsets that have had the greatest impact on numerous fields of l...
If one considers chemical-biology toolsets that have had the greatest impact on numerous fields of l...