Development of a nanostructured electrochemical immunosensor applied to the early detection of invasive aspergillosis
- Piguillem Palacios, Sofía Viviana
- Ortega Sánchez, Francisco Gabriel
- Raba, Julio
- Messina, Germán Alejandro
- Fernández Baldo, Martín Alejandro
DOIAbstract
The use of a biological marker as an adjunct for screening for invasive aspergillosis (IA) in high-risk patients is attractive, because it is noninvasive and may detect evidence of IA prior to the appearance of clinical signs and symptoms. Galactomannan (GMN) is a polysaccharide cell-wall component that is released by Aspergillus species. In the present work, an electrochemical microfluidic immunosensor for GMN detection was developed. Moreover, copper nanoparticles covered with polyvinylpyrrolidone (CuNPs-PVP) were synthesized, characterized and used in a microfluidic immunosensor based on the use of anti-GMN antibodies as a trapping agent. The concentration of trapped GMN is then electrochemically quantified by HRP-conjugated anti-GMN-ant...
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