Enzymatic and chemical synthesis of new anticoagulant peptides
- Origone, Anabella Lucía
- Bersi, Grisel
- Illanes, Andrés
- Sturniolo, Héctor Luis
- Liggieri, Constanza Silvina
- Guzmán, Fanny
- Barberis, Sonia Esther
DOIAbstract
In this study we report the enzymatic synthesis of N-α-[Carbobenzyloxy]-Tyr-Gln-Gln (Z-YQQ), a new anticoagulant tripeptide. It was obtained using phytoproteases from the stems and petioles of Asclepias curassavica L. as catalyst in an aqueous–organic biphasic system formed by 50% (v/v) ethyl acetate and 0.1 M Tris–HCl buffer pH 8. The resulting peptide was compared with the analogous peptide Tyr-Gln-Gln (YQQ) produced by solid-phase chemical synthesis. The in vitro anticoagulant activity of the aforementioned peptides was determined using Wiener Lab Test (Wiener, Argentina). The toxicological activity of the peptides was also determined. The enzymatically synthesized Z-YQQ peptide acted on the extrinsic pathway of the coagulation cascade, ...
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