Immunogenetic features in patients with chronic hepatitis C

The aim of this study was to investigate the gene IL-28B polymorphism and indices of the cellular immunity in patients with chronic hepatitis C (CHC) and healthy individuals belonging to ethnically homogeneous group of residents of Odessa region as well as to determine possible association of certain genotypes and the degree of liver fibrosis. Polymorphism was investigated using corresponding portions of the genome by PCR. The structure of the primers used and the parameters of temperature cycles are described in the literature and genomic database. Evaluation of morphological changes in the liver (the degree of activity and severity of fibrotic changes) was determined by the scale METAVIR using a non-invasive method Fibrotest. There was revealed a low inverse correlation between genotypes of IL-28B and the degree of liver fibrosis