Coumarins and alkaloids in shoot culture of Ruta graveolens L.

A shoot culture of Ruta graveolens L. (Rutaceae) was maintained in the stationary liquid phase. From the cultured shoots seven compounds were isolated and identified as psoralen, bergapten, xanthotoxin, isopimpinellin (linear furanocoumarins), rutamarin (linear dihydrofuranocoumarin), kokusaginine and skimmianine (furanoquinoline alkaloids) by spectral methods. The compounds are known as secondary metabolites of the intact plant, as well as its cell and tissue cultures