Mycoflora of Indoor Dust and Alternaria Alternata Growth on Building Materials

Alternaria alternata, a clinically significant mold species is associated with allergic disease and asthma. An indoor growth of A. alternata may initiate atopic symptoms, accumulate in dusts, and discolor and deteriorate indoor building materials. Environmental control should be the initial approach to maintain a healthy indoor environment and manage allergic diseases. The current thesis investigated indoor dusts from various environments for mold spores and Alt a 1. Laboratory studies were carried out to determine if or which commercially available mold-resistant treatments and building materials could efficiently decrease A. alternata growth and Alt a 1. The mycoflora of indoor dusts was microscopically examined and the clinically significant allergen from A. alternata, Alt a 1 was measured in sampled dusts using a monoclonal anti-recombinant Alt a 1 (rAlt a 1) enzyme linked immuno-sorbent assay (ELISA). To investigate building material susceptibility untreated, mildew-resistant painted, and commercially available biocide treated building materials were inoculated with A. alternata spores, incubated in a humidity controlled chamber, and compared for mold growth and the presence of Alt a 1. A photo-monitoring technique was used to survey and determine mold growth surface area coverage. Alt a 1 immuno-reactivity of superficial mold growths was analyzed with the ELISA. The microscopic speciation of mold spores indicated that a diverse mycoflora can be found in indoor dusts. Although A. alternata was found in low concentrations in a few samples, Alt a 1was below the limit of detection. Nevertheless, mold spores can significantly contribute to the complexity of indoor dusts. A. alternata grew on all tested building materials but both painted and treated building materials inhibited mold growth in comparison to untreated samples. Not all material surfaces contained detectable levels of Alt a 1. Comparisons between untreated and painted building material groups indicated that a significant difference between the Alt a 1 accumulation content could not be established. To establish a standard sampling protocol and reliable prevention methods for mold contamination and exposure more knowledge about indoor molds must be gained.M.S. in Environmental Engineering, December 201