UJI SITOTOKSISITAS EKSTRAK METANOL UMBI BIT (Beta vulgaris L. var. rubra L.) TERHADAP CELL LINE T47D

ABSTRACTTherapy failure of breast cancer as a consequence of cell resistention has encouraged the effort to develop a cancer medicine including herbal medicine. Based on some research, it can be concluded that there is antioxydant and anticancer activity from beetroot involved with betasianin and betasantin substance that belong to flavonoid compound. The purpose of this research was to find the effect cytotoxic of methanolic extract of beetroot (Beta vulgaris L. var. rubra L.) on cell line T47D which was a breast cancer cell.This research was conducted on several experiment step namely plant determination, cell preparation, cell harvest, methanolic extraction, and cytotoxic test. The extraction of beetroot is conducted by maceration method using methanol 70 %. Cytotoxic test on cell line T47D is conducted by MTT assay using five concentration ceries were 500, 250, 125, 62,5 and 31,25 ug/ml. Data analysis uses probit analysis.Research result shows that methanolic extract of beetroot there was no cytotoxic activity on cell line T47D. It shows that five consentration series methanolic extraction of beetroot have % cell viability more than 100%.Keyword: Beetroot, Beta vulgaris L. var. rubra L, Cell line T47D, Cytotoxic, Flavonoid