Occurrenceo f Iris mild mosaicp otyvirus in cultivatedi ris in India

Iris (Iris x hollandica Hort. cv. Bluemagic)p lants showing mosaic symptomsw ere testedf or the presenceo f Iris mild mosaic polyvirus (IMMV) by host range, enzyme linked immunosorbent assay (ELISA) using antibodies specific for IMMV), reverset ranscriptionp olymerasec hain reaction (RT-PCR),i mmunocapturere verset ranscriptionp olymerasec hain reaction (IC-RT -PCR) using potyvirus group specific primers and antibodies specific to IMMV, immune electron microscopy and cytopathology. Presence of IMMV was detected by ELISA, confirmed by RT-PCR, IC-RT-PCR, IEM, cytopathologya nd sequencingo f the cloned PCR product. From the sequenceo f the virus, specific primers were designed and specific detection of IMMV from iris was standardize